0
January 1975, Vol 111, No. 1 >
Article | January 1975

Production of Sézary-like Cells From Normal Human Lymphocytes

James A. Yeckley, MD; William L. Weston, MD; E. George Thorne, MD; Gerald G. Krueger, MD
[+] Author Affiliations

Accepted for publication Aug 29, 1974.

Reprint requests to the Division of Dermatology, University of Colorado Medical Center, 4200 E Ninth Ave, Denver, CO 80220 (Dr. Weston).


From the Division of Dermatology, University of Colorado School of Medicine, Denver (Drs. Yeckley, Weston, and Thorne), and the Division of Dermatology, University of Utah School of Medicine, Salt Lake City (Dr. Krueger).


Arch Dermatol. 1975;111(1):29-32. doi:10.1001/archderm.1975.01630130031001
Text Size: A A A
Published online
Article
References
Comments

The present study was designed to determine if lymphocytes from healthy humans could be stimulated to assume the morphologic appearance of Sézary-like cells.

Lymphocyte-rich populations of cells were incubated for 72 hours with pokeweed mitogen and phytohemagglutinin, both potent cellular mitogens. With light microscopy, differential cell counts performed on 0.5μ epoxy resin-embedded sections showed that 5% to 11% of the lymphocytes had cerebriform nuclei, and were designated as Sézary-like cells. The morphological results were confirmed by electron microscopy.

Production of Sézary-like cells from stimulated, normal, human lymphocytes suggests that cells with cerebriform nuclei may represent reactive lymphocytes, and may explain their presence in benign inflammatory dermatoses.

REFERENCES

1 +
Lutzner NA, Jordon HW:  The ultrastructure of an abnormal cell in Sézary's syndrome . Blood 31:719-726, 1968;.
2 +
Taswell HT, Winkelmann RK:  Sézary syndrome: A malignant reticulemic erythroderma . JAMA 177:465-472, 1961;.
3 +
Orbaneja JG, Yus ES, Diaz-Flores L, et al:  Cytology of the mycosis fungoides and the Sézary syndrome: Correlation between light and electron microscopy . Br J Dermatol 87:96-105, 1972;.
4 +
Flaxman BA, Zelazny G, Van Scott EJ:  Nonspecificity of characteristic cell in mycosis fungoides . Arch Dermatol 104:141-147, 1971;.
5 +
Flaxman BA, Van Scott EJ, Goldman LI:  Sézary-like cells in cultures of normal human skin . Arch Dermatol 109:577-578, 1974;.
6 +
Douglas SD, Cohnen G, Brittinger G:  Ultrastructural comparison between phytomitogen transformed normal and chronic lymphocytic leukemia lymphocytes . J Ultrastruct Res 44:11-26, 1973;.
7 +
Lutzner MA, Hobbs JW, Horvath P:  Ultrastructure of abnormal cells: In Sézary syndrome, mycosis fungoides, and parapsoriasis en plaque . Arch Dermatol 103:375-386, 1971;.
8 +
Boyum A:  Isolation of leucocytes from human blood . Scand J Clin Lab Invest 21( (suppl 97) ):9-29, 1968;.
9 +
Nichols BA, Bainton DF, Farquhar MG:  Differentiation of monocytes: Origin, nature and fate of their azurophil granules . J Cell Biol 50:498-515, 1971;.
10 +
Crossen PE, Mellor JEL, Finley AG, et al:  The Sézary syndrome: Cytogenetic studies and identification of the Sézary cell as an abnormal lymphocyte . Am J Med 50:24-34, 1971;.
11 +
Brouet J-C, Flandrin G, Seligmann M:  Indications of thymus-derived nature of the proliferating cells in six patients with Sézary's syndrome . N Engl J Med 289:341-344, 1973;.
12 +
Lutzner MA, Edelson RS, Smith RW, et al:  Two varieties of Sézary syndrome, both bearing T-cell markers . Lancet 2:207, 1973;.
13 +
Winkelmann RK:  T cell erythroderma: (Sézary syndrome) . Arch Dermatol 108:205-206, 1973;.
14 +
Winkelmann RK, Linman JW:  Erythroderma with atypical lymphocytes (Sézary syndrome) . Am J Med 55:192-198, 1973;.
15 +
Douglas SD, Hoffman JB, Borjeson J, et al:  Studies on human peripheral blood lymphocytes in vitro: III. Fine structural features of lymphocyte transformation by pokeweed mitogen . J Immunol 98:17-30, 1967;.
16 +
Biberfeld P:  Morphogenesis in blood lymphocytes stimulated with phytohaemagglutinin (PHA): A light and electron microscopic study . Acta Pathol Microbiol Scand [A] 223( (suppl) ):1-70, 1971;.

Figures

Tables

Interactive Graphics

Video

Country-Specific Mortality and Growth Failure in Infancy and Yound Children and Association With Material Stature

Use interactive graphics and maps to view and sort country-specific infant and early dhildhood mortality and growth failure data and their association with maternal

1 +
Lutzner NA, Jordon HW:  The ultrastructure of an abnormal cell in Sézary's syndrome . Blood 31:719-726, 1968;.
2 +
Taswell HT, Winkelmann RK:  Sézary syndrome: A malignant reticulemic erythroderma . JAMA 177:465-472, 1961;.
3 +
Orbaneja JG, Yus ES, Diaz-Flores L, et al:  Cytology of the mycosis fungoides and the Sézary syndrome: Correlation between light and electron microscopy . Br J Dermatol 87:96-105, 1972;.
4 +
Flaxman BA, Zelazny G, Van Scott EJ:  Nonspecificity of characteristic cell in mycosis fungoides . Arch Dermatol 104:141-147, 1971;.
5 +
Flaxman BA, Van Scott EJ, Goldman LI:  Sézary-like cells in cultures of normal human skin . Arch Dermatol 109:577-578, 1974;.
6 +
Douglas SD, Cohnen G, Brittinger G:  Ultrastructural comparison between phytomitogen transformed normal and chronic lymphocytic leukemia lymphocytes . J Ultrastruct Res 44:11-26, 1973;.
7 +
Lutzner MA, Hobbs JW, Horvath P:  Ultrastructure of abnormal cells: In Sézary syndrome, mycosis fungoides, and parapsoriasis en plaque . Arch Dermatol 103:375-386, 1971;.
8 +
Boyum A:  Isolation of leucocytes from human blood . Scand J Clin Lab Invest 21( (suppl 97) ):9-29, 1968;.
9 +
Nichols BA, Bainton DF, Farquhar MG:  Differentiation of monocytes: Origin, nature and fate of their azurophil granules . J Cell Biol 50:498-515, 1971;.
10 +
Crossen PE, Mellor JEL, Finley AG, et al:  The Sézary syndrome: Cytogenetic studies and identification of the Sézary cell as an abnormal lymphocyte . Am J Med 50:24-34, 1971;.
11 +
Brouet J-C, Flandrin G, Seligmann M:  Indications of thymus-derived nature of the proliferating cells in six patients with Sézary's syndrome . N Engl J Med 289:341-344, 1973;.
12 +
Lutzner MA, Edelson RS, Smith RW, et al:  Two varieties of Sézary syndrome, both bearing T-cell markers . Lancet 2:207, 1973;.
13 +
Winkelmann RK:  T cell erythroderma: (Sézary syndrome) . Arch Dermatol 108:205-206, 1973;.
14 +
Winkelmann RK, Linman JW:  Erythroderma with atypical lymphocytes (Sézary syndrome) . Am J Med 55:192-198, 1973;.
15 +
Douglas SD, Hoffman JB, Borjeson J, et al:  Studies on human peripheral blood lymphocytes in vitro: III. Fine structural features of lymphocyte transformation by pokeweed mitogen . J Immunol 98:17-30, 1967;.
16 +
Biberfeld P:  Morphogenesis in blood lymphocytes stimulated with phytohaemagglutinin (PHA): A light and electron microscopic study . Acta Pathol Microbiol Scand [A] 223( (suppl) ):1-70, 1971;.

Correspondence

Submit a Letter
CME Course for:


You need to register in order to view this quiz.


To understand the clinical management of acute heart failure syndromes.
Accreditation Information The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.
The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity.
Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Note: You must get at least of the answers correct to pass this quiz.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.
To view and print your certificate and access a summary of your CME courses go to My CME.
NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s “Cited By” API will populate this tab (http://www.crossref.org/citedby.html).
Compression-Only CPR: Pushing the Science Forward
Cone
AAM 2010;304:1493-1495.
All you need to read in the other general journals
BMJ 2010;341:c5893-c1494.
Submit a Response

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

© 2012 American Medical Association. All Rights Reserved.
Powered bySilverchair Information Systems