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IgG and Complement-Fixing Pseudointercellular Antibodies After Thermal Injury

Richard J. Ablin, PhD; Ian Alan Holder, PhD
[+] Author Affiliations

Accepted for publication Dec 28, 1973.

Reprint requests to Immunobiology Section, Division of Urology, Cook County Hospital, Chicago, IL 60612 (Dr. Ablin).


Chicago; Cincinnati

From the Cook County Hospital and Graduate School of Medicine, the Hektoen Institute and the Chicago Medical School University of Health Sciences, Chicago (Dr. Ablin), and the University of Cincinnati College of Medicine and the Shriners Burn Institute, Cincinnati (Dr. Holder).


Arch Dermatol. 1974;109(6):887-888. doi:10.1001/archderm.1974.01630060063018
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The IgG and complement-fixing (C'F) peripheral or membranal antinuclear autoantibodies masquerading as intercellular antibodies have been observed by indirect immunofluorescence on autologous skin biopsy specimens of two patients following thermal injury. Immunohistologic localization in these patients of in vivo bound C' to the nuclear membrane, the same site at which in vitro reactions of both IgG and C'F autoantibody occur offer further evidence suggestive of the participation of immunologic phenomena following thermal injury that may contribute to the generalized depression of host resistance in burn patients.

REFERENCES

Thivolet J, Beyvin AJ:  Recherche par immunofluorescence d' auto anticorps sériques via á vis des constituants de l' épidermo chez les brûlés . Experientia 24:945-946, 1968;.
Ablin RJ, et al:  Pemphigus-like antibodies in patients with skin burns . Vox Sang 16:73-75, 1969;.
Quismorio FP, Bland SL, Friou GJ:  Autoimmunity in thermal injury: Occurrence of rheumatoid factors, antinuclear antibodies and antiepithelial antibodies . Clin Exp Immunol 8:701-711, 1971;.
Burnham TK, Fine GB:  Indirect cutaneous immunofluorescence. 1. Morphologic observations in bullous disease . Arch Dermatol 105:52-58, 1972;.
Beutner EH, Sepulveda MR, Barnett EV:  Quantitative studies of immunofluorescent staining . Bull WHO 39:587-606, 1968;.
Beutner EH, Chorzelski TP, Jordon RE:  Methods of immunofluorescent staining for pemphigus and bullous pemphigoid antibodies , in Beutner EH, Chorzelski TP, Jordon RE (eds): Autosensitization in Pemphigus and Bullous Pemphigoid. Springfield , Ill, Charles C Thomas Publisher, 1971;, pp 170-172.
Lachman PJ, et al:  The localization of in vivo bound complement in tissue sections . J Exp Med 116:63-82, 1962;.
Coons AH, Kaplan MH:  Localization of antigen in tissue cells: II. Improvements in the method for detection of antigen by means of fluorescent antibody . J Exp Med 91:1-13, 1950;.
Tojo T, Friou GJ:  Lupus nephritis: Varying complement-fixing properties of immunoglobulin G antibodies to antigens of cell nuclei . Science 161:903-906, 1968;.
Borsos T, Rapp HJ:  Complement fixation on cell surfaces by 19S and 7S antibodies . Science 150:505-506, 1965;.
Pike RM:  Antibody heterogeneity and serological reactions . Bacteriol Rev 31:157-174, 1967;.
Ablin RJ:  Antiepithelial antibodies in the sera of patients with benign and malignant diseases of the prostate . Z Immunitaetforsch 146:8-22, 1973;.

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Thivolet J, Beyvin AJ:  Recherche par immunofluorescence d' auto anticorps sériques via á vis des constituants de l' épidermo chez les brûlés . Experientia 24:945-946, 1968;.
Ablin RJ, et al:  Pemphigus-like antibodies in patients with skin burns . Vox Sang 16:73-75, 1969;.
Quismorio FP, Bland SL, Friou GJ:  Autoimmunity in thermal injury: Occurrence of rheumatoid factors, antinuclear antibodies and antiepithelial antibodies . Clin Exp Immunol 8:701-711, 1971;.
Burnham TK, Fine GB:  Indirect cutaneous immunofluorescence. 1. Morphologic observations in bullous disease . Arch Dermatol 105:52-58, 1972;.
Beutner EH, Sepulveda MR, Barnett EV:  Quantitative studies of immunofluorescent staining . Bull WHO 39:587-606, 1968;.
Beutner EH, Chorzelski TP, Jordon RE:  Methods of immunofluorescent staining for pemphigus and bullous pemphigoid antibodies , in Beutner EH, Chorzelski TP, Jordon RE (eds): Autosensitization in Pemphigus and Bullous Pemphigoid. Springfield , Ill, Charles C Thomas Publisher, 1971;, pp 170-172.
Lachman PJ, et al:  The localization of in vivo bound complement in tissue sections . J Exp Med 116:63-82, 1962;.
Coons AH, Kaplan MH:  Localization of antigen in tissue cells: II. Improvements in the method for detection of antigen by means of fluorescent antibody . J Exp Med 91:1-13, 1950;.
Tojo T, Friou GJ:  Lupus nephritis: Varying complement-fixing properties of immunoglobulin G antibodies to antigens of cell nuclei . Science 161:903-906, 1968;.
Borsos T, Rapp HJ:  Complement fixation on cell surfaces by 19S and 7S antibodies . Science 150:505-506, 1965;.
Pike RM:  Antibody heterogeneity and serological reactions . Bacteriol Rev 31:157-174, 1967;.
Ablin RJ:  Antiepithelial antibodies in the sera of patients with benign and malignant diseases of the prostate . Z Immunitaetforsch 146:8-22, 1973;.

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