Agar media, for a variety of mycologic laboratory purposes, were for many years prepared in the familiar agar slants in glass tubes plugged with cotton. Storage of these prepared glass tube slants is unsatisfactory because the agar medium dries up within several weeks. It is therefore difficult to keep such slants ready for the occasional use in a doctor's office laboratory.
For some time now, various types of tightly capped glass and plastic tubes, bottles, and plates have been prepared. These are completely satisfactory for good laboratory work. They have good shelf life and offer additional advantages such as compactness, easy legibility of labels, and are less apt to dry out after inoculation than the cotton plugged tubes. Culture colonies 1 to 2 weeks old show good morphological characteristics. Pathogenic colonies of this age are often about 1 cm. in diameter. Therefore, the usual 8 cm. Petri dishsize areas are