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Research Letter |

Increased IL-23 Expression in Palmoplantar Psoriasis and Hyperkeratotic Hand Dermatitis FREE

Joseph V. Lillis, MD; Chang-Sheng Guo, PhD; Jennifer J. Lee, MD; Andrew Blauvelt, MD
[+] Author Affiliations

Author Affiliations: Division of Dermatology, University of California, San Diego Medical Center, La Jolla (Dr Lillis); Departments of Dermatology (Drs Lillis, Lee, and Blauvelt) and Molecular Microbiology & Immunology (Dr Blauvelt), Oregon Health and Science University, and Dermatology Service, Veterans Affairs Medical Center (Drs Guo and Blauvelt), Portland.


Arch Dermatol. 2010;146(8):918-935. doi:10.1001/archdermatol.2010.168.
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Published online

Patients with hyperkeratotic hand dermatitis (HHD) have chronic, sharply demarcated, fissure-prone plaques on the palms and/or soles without evidence of other skin diseases such as atopic dermatitis or psoriasis.1 Although HHD has a distinct clinical picture, the histologic pattern of HHD is nonspecific, showing “spongiotic psoriasiform” type changes.1 The cause of HHD is unknown, and whether it is a chronic eczematous process or a variant of localized psoriasis is controversial.

Recent evidence suggests that type 17 helper T (TH17) cells, which are CD4+ T cells that produce interleukin (IL) 17A, are major contributors to psoriasis pathogenesis.2 Characteristically, TH17 cells express IL-23 receptors, which makes them responsive to IL-23, a cytokine that promotes survival and proliferation of TH17 cells.2 Although chronic psoriatic plaques have demonstrated elevated levels of IL-23, IL-23 expression has not been evaluated in palmoplantar psoriasis or HHD. Thus, we sought to compare IL-23 expression patterns in chronic plaque psoriasis, palmoplantar psoriasis, and HHD.

The Oregon Health and Science University institutional review board approved all aspects of this study, and informed consent was obtained from each participant. Patients were diagnosed as having HHD if they demonstrated well-demarcated, symmetric, fissure-prone, painful, indurated, and hyperkeratotic plaques on the palms with a sharp cutoff at the wrist. In addition, there could be no personal or family history of typical chronic plaque psoriasis lesions elsewhere on the body, no history of atopic dermatitis, and no relevant positive findings on patch testing. Patients were considered to have palmoplantar psoriasis if they presented with well-demarcated, symmetric, indurated, and hyperkeratotic plaques on the palms with a sharp cutoff at the wrist. Patients were considered to have typical chronic plaque psoriasis if lesions were distributed on the extensor elbows, knees, scalp, nails, or trunk. All patients had chronic disease present for at least 6 months and all had not had topical therapy for at least 2 weeks and systemic therapy for at least 4 weeks.

Tissue samples were obtained by 4-mm punch biopsy specimens taken from lesional skin of patients with HHD (n = 4), palmoplantar psoriasis (n = 4), and chronic plaque psoriasis (n = 6) and nonlesional skin of patients with psoriasis (n = 6). Frozen tissue sections were prepared, and slides were incubated in 10% methanol for 10 minutes, in 3% hydrogen peroxide for 10 minutes, and then in blocking solution for 1 hour. Antibodies directed against human IL-23 (Biolegend, San Diego, California) were then applied at a concentration of 1:50 overnight at 4°C. Vectastain Elite ABC-Peroxidase kits and Vector VIP peroxidase substrate kits (Vector Laboratories, Burlingame, California) were used for visualizing cells. Positive cells were counted in 8 high-power fields, and the average number of labeled cells was calculated. A 1-way, between-subject analysis of variance was conducted to compare the expression level of IL-23 in the 4 groups. A paired t test was used in post hoc analysis to individually compare each of the 4 groups against one another.

There was a significant difference in the number of IL-23–positive cells in the 4 groups of patients (F3,16 = 6.68) (P < .05 for each group). Post hoc comparisons showed markedly increased IL-23–staining cells in HHD, palmoplantar psoriasis, and lesional psoriasis compared with nonlesional psoriasis (Figure and Table) (P < .05 for each disease type). There were no significant differences in the number of IL-23–positive cells between the 3 disease groups (data not shown). Positively stained cells were found in the tips of the dermal papilla, and they demonstrated dendritic morphologic characteristics (Figure).

Place holder to copy figure label and caption
Figure.

Biopsy specimens from study patients. A-D, Representative interleukin 23 immunohistochemical analysis of palmoplantar psoriasis (A), hyperkeratotic hand dermatitis (B), chronic lesional plaque psoriasis from the body (C), and nonlesional psoriasis from the body (D). E, Representative isotype control background staining in chronic lesional plaque psoriasis from the body. Scale bars in all panels represent 50 μm. Vector VIP peroxidase substrate kits were used for antibody visualization (Vector Laboratories, Burlingame, California), and the counterstain was hematoxylin-eosin.

Graphic Jump Location
Table Graphic Jump LocationTable. Comparison of IL-23–Positive Immunohistochemical Reactions Across Studied Diseases

Our data demonstrate that IL-23 is upregulated in both HHD and palmoplantar psoriasis, similar to what has been previously reported in chronic plaque psoriasis.2 Palmoplantar psoriasis and HHD are diseases associated with considerable morbidity and are often difficult to treat.1,3 Topical therapy is notoriously inadequate, and patients often move to other therapies such as acitretin or biological agents with varying success.3 Ustekinumab is a new biological drug that targets the shared p40 subunit of IL-23 and IL-12 and has shown excellent clinical responses in patients with chronic plaque psoriasis.4,5 We predict that by targeting IL-23, these agents may be effective in individuals with HHD or palmoplantar psoriasis.

ARTICLE INFORMATION

Correspondence: Dr Lillis, Division of Dermatology, 9350 Campus Point Dr, MC#0975, University of California, San Diego Medical Center, La Jolla, CA 92037 (jlillis@ucsd.edu).

Accepted for Publication: February 6, 2010.

Author Contributions: Drs Lillis and Blauvelt had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis. Study concept and design: Lillis and Blauvelt. Acquisition of data: Lillis, Guo, Lee, and Blauvelt. Analysis and interpretation of data: Lillis and Blauvelt. Drafting of the manuscript: Lillis, Guo, Lee, and Blauvelt. Critical revision of the manuscript for important intellectual content: Blauvelt. Statistical analysis: Blauvelt. Obtained funding: Blauvelt. Administrative, technical, and material support: Lillis, Guo, Lee, and Blauvelt. Study supervision: Blauvelt.

Financial Disclosure: Dr Blauvelt is a consultant and investigator for Abbott and Centocor and has consulted for Lilly. He has also received honoraria from Abbott, Centocor, and Lilly.

Funding/Support: This research was supported in part by a Veterans Affairs Merit Award and National Institutes of Health grant 1 R21 AR054495-01A1 (Dr Blauvelt).

Additional Contributions: Frances J. Storrs, MD, helped to clinically defined HHD for this study and provided patients for the HHD cohort.

Hersle  KMobacken  H Hyperkeratotic dermatitis of the palms. Br J Dermatol 1982;107 (2) 195- 201
PubMed Link to Article
Di Cesare  ADi Meglio  PNestle  FO The IL-23/Th17 axis in the immunopathogenesis of psoriasis. J Invest Dermatol 2009;129 (6) 1339- 1350
PubMed Link to Article
Thestrup-Pedersen  KAndersen  KEMenne  TVeien  NK Treatment of hyperkeratotic dermatitis of the palms (eczema keratoticum) with oral acitretin: a single-blind placebo controlled study. Acta Derm Venereol 2001;81 (5) 353- 355
PubMed Link to Article
Leonardi  CLKimball  ABPapp  KA  et al. PHOENIX 1 study investigators, Efficacy and safety of ustekinumab, a human interleukin-12/23 monoclonal antibody, in patients with psoriasis: 76-week results from a randomised, double-blind, placebo-controlled trial (PHOENIX 1). Lancet 2008;371 (9625) 1665- 1674
PubMed Link to Article
Papp  KALangley  RGLebwohl  M  et al. PHOENIX 2 study investigators, Efficacy and safety of ustekinumab, a human interleukin-12/23 monoclonal antibody, in patients with psoriasis: 52-week results from a randomised, double-blind, placebo-controlled trial (PHOENIX 2). Lancet 2008;371 (9625) 1675- 1684
PubMed Link to Article

Figures

Place holder to copy figure label and caption
Figure.

Biopsy specimens from study patients. A-D, Representative interleukin 23 immunohistochemical analysis of palmoplantar psoriasis (A), hyperkeratotic hand dermatitis (B), chronic lesional plaque psoriasis from the body (C), and nonlesional psoriasis from the body (D). E, Representative isotype control background staining in chronic lesional plaque psoriasis from the body. Scale bars in all panels represent 50 μm. Vector VIP peroxidase substrate kits were used for antibody visualization (Vector Laboratories, Burlingame, California), and the counterstain was hematoxylin-eosin.

Graphic Jump Location

Tables

Table Graphic Jump LocationTable. Comparison of IL-23–Positive Immunohistochemical Reactions Across Studied Diseases

References

Hersle  KMobacken  H Hyperkeratotic dermatitis of the palms. Br J Dermatol 1982;107 (2) 195- 201
PubMed Link to Article
Di Cesare  ADi Meglio  PNestle  FO The IL-23/Th17 axis in the immunopathogenesis of psoriasis. J Invest Dermatol 2009;129 (6) 1339- 1350
PubMed Link to Article
Thestrup-Pedersen  KAndersen  KEMenne  TVeien  NK Treatment of hyperkeratotic dermatitis of the palms (eczema keratoticum) with oral acitretin: a single-blind placebo controlled study. Acta Derm Venereol 2001;81 (5) 353- 355
PubMed Link to Article
Leonardi  CLKimball  ABPapp  KA  et al. PHOENIX 1 study investigators, Efficacy and safety of ustekinumab, a human interleukin-12/23 monoclonal antibody, in patients with psoriasis: 76-week results from a randomised, double-blind, placebo-controlled trial (PHOENIX 1). Lancet 2008;371 (9625) 1665- 1674
PubMed Link to Article
Papp  KALangley  RGLebwohl  M  et al. PHOENIX 2 study investigators, Efficacy and safety of ustekinumab, a human interleukin-12/23 monoclonal antibody, in patients with psoriasis: 52-week results from a randomised, double-blind, placebo-controlled trial (PHOENIX 2). Lancet 2008;371 (9625) 1675- 1684
PubMed Link to Article

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