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Regression of Deeply Infiltrating Giant Condyloma (Buschke-Löwenstein Tumor) Following Long-term Intralesional Interferon Alfa Therapy FREE

Alexandra Geusau, MD; Gertraud Heinz-Peer, MD; Beatrix Volc-Platzer, MD; Georg Stingl, MD; Reinhard Kirnbauer, MD
[+] Author Affiliations

Section Editor: George J. Hruza, MD
Assistant Section Editor: Lynn A. Cornelius, MD
Assistant Section Editor: Jon Starr, MD

More Author Information
Arch Dermatol. 2000;136(6):707-710. doi:10.1001/archderm.136.6.707.
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In 1995, a 40-year-old heterosexual man developed a perianal tumor that rapidly increased in size. Following a clinical and histologic diagnosis of a large condyloma acuminatum (CA), the tumor was partially resected in a primary care hospital. Subsequently, recurrent perianal fistulae and abscesses required several surgical interventions, and the patient underwent a transverse colostomy to allow subsequent complete resection of the condyloma.

At the patient's first visit in our clinic in 1996, physical examination demonstrated a smooth, exophytic, cauliflowerlike perianal verrucous tumor extending toward the perineum, scrotum, and buttocks (Figure 1, left). A magnetic resonance imaging scan revealed that the tumor infiltrated deeply into the right side of the pelvis, including the ischiorectal fossa, internal obturator muscle, anal sphincter, perineum, and penile root (Figure 2, top). Histologic reevaluation of a previously obtained biopsy specimen showed a large papillomatous tumor infiltrating deeply into the underlying tissues, without penetration of the basement membrane (Figure 3, top). The epithelium was acanthotic with blunt rete ridges, well stratified without apparent cellular atypia, and contained few mitoses and koilocytes (Figure 3, bottom). There was no evidence of areas of malignant degeneration or squamous cell carcinoma (SCC). DNA was extracted from the biopsy specimen and analyzed for human papillomavirus (HPV) DNA by hybrid capture assay (Digene; Abbott Diagnostics, Silver Spring, Md). The sample was positive for low-risk HPV type(s) and identified as HPV-6b by consensus primer polymerase chain reaction,1 followed by DNA sequencing of the amplimer (data not shown). Taken together, these findings were consistent with the diagnosis of giant condyloma (GC), or Buschke-Löwenstein tumor. The results of routine laboratory examinations were normal; serologic tests were negative for syphilis and human immunodeficiency virus; and lymphocyte subpopulations were normal.

Place holder to copy figure label and caption
Figure 1.

Left, Clinical presentation. A large, exophytic, cauliflowerlike perianal verrucous tumor is seen extending toward the perineum and scrotum. Right, Dramatic regression of the tumor is evident after 12 months of treatment.

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Place holder to copy figure label and caption
Figure 2.

Magnetic resonance imaging scans (short-time inversion recovery technique). Top, Before treatment, the tumor is seen infiltrating the internal obturator muscle, ischiorectal fossa, and perirectal space (arrows indicate the lateral tumor border). Bottom, After 12 months of treatment, the internal obturator muscle is again well defined (arrow).

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Place holder to copy figure label and caption
Figure 3.

Top, Pretreatment histologic section shows papillomatous tumor invading deeply into the underlying tissue, without penetration of the basement membrane (hematoxylin-eosin, original magnification ×200). Bottom, The epidermis is hyperplastic and well stratified with few mitoses or koilocytes (hematoxylin-eosin, original magnification ×400).

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The primary therapeutic option of GC is radical surgery with or without adjunctive chemotherapy.2,3 Owing to the large extent of tumor invasion in this case, curative surgery would have been achieved only by hemipelvectomy and limb amputation. This severe mutilation was refused by the patient. Thus, alternative strategies had to be considered.

Systemic or intralesional treatment with interferon resulted in complete remission in 2 previously described cases of Buschke-Löwenstein tumor,4,5 but failed to result in improvement in others.6,7 Because of the cases that showed remission, treatment with intralesional injections with interferon alfa-2b (Intron A; Schering Corp, Kenilworth, NJ) was initiated. The injections were well tolerated, without significant adverse effects, and the dosage was increased rapidly to 10 million units 3 times a week and continued on an outpatient basis. During the next few months, the tumor did not change clinically. However, about 6 months after the interferon therapy was initiated, the tumor slowly decreased in size. After 9 months of continuous treatment, clinical examination and magnetic resonance imaging revealed dramatic tumor regression, which appeared to be complete after approximately 12 months, leaving a deep defect in the right ischiorectal fossa (Figure 1, right, and Figure 2, bottom). To ensure sustained remission, treatment (10 million units 3 times a week) was continued for an additional 16 months and was then discontinued. Currently, after 28 months of treatment and 4 months of observation, no recurrence could be seen on magnetic resonance imaging scans. The absence of tumor was best demonstrated in short-time inversion recovery sequences, in which tumor tissue is displayed brightly against a dark background, allowing differentiation between active (with increased water content) and healed lesions.8

A second biopsy specimen, which was obtained 12 months after the interferon alfa-2b therapy was initiated, revealed the absence of residual tumor tissue. Strikingly, a dense bandlike mononuclear cell infiltrate was seen closely attached to and invading the epithelium, as is seen in lichen planus (Figure 4). The majority of the cells were CD3- and CD4-positive T lymphocytes; a minority of the cells were CD8-positive T cells; and a few CD68-positive macrophages were present. Single cells in the basal epithelial layers displayed discrete staining with an antibody to perforin. Also, a significant number of epithelial cells were positive for CD54 (intercellular adhesion molecule 1) and/or HLA-DR, molecules that are important for the initiation of immune responses. A DNA extract was negative for HPV by hybrid capture test and polymerase chain reaction.

Place holder to copy figure label and caption
Figure 4.

Histologic section of residual tissue after 12 months of treatment shows a bandlike mononuclear infiltrate in close approximation to and invading into the epidermis (hematoxylin-eosin, original magnification ×100).

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Giant condylomata were first described by Buschke and Löwenstein9 in 1925 as "carcinomalike condylomata acuminata of the penis." The GC, or Buschke-Löwenstein tumor, is a semimalignant neoplasm of the external genitalia and the perianal region. In contrast to CA, GC is characterized by locally invasive growth, resulting in local complications. Initially, it may be misdiagnosed as benign CA or malignant SCC. The tumor has a tendency to recur and to form abscesses and fistulae when it is present in a perianal area.10 The GC is part of a group of tumors designated verrucous carcinomas, which include florid oral papillomatosis of Ackerman (oropharyngeal), epithelioma cuniculatum (palmoplantar), and papillomatosis cutis carcinoides of Gottron (cutaneous).11 It is a rare condition, and its pathogenesis and natural history are not well understood. It has been proposed that GC represents an intermediate state between CA and SCC.12 Presumably, for unknown reasons, a very small subset of long-lasting CA eventually evolve into slowly invading tumors and then, if left untreated, into large papillomatous proliferations that penetrate deeply into the underlying tissue.10 A characteristic of GC is its benign-appearing histologic appearance, which resembles that of CA. It may be difficult to distinguish between these 2 conditions, particularly at an early stage of the disease. A large representative biopsy specimen is crucial to judge the architecture of the lesion in order to establish the diagnosis and to rule out foci of SCC. Giant condylomata invade by expansion rather than by infiltration, leaving the basement membrane intact, and show a well-stratified epithelium with minimal cellular dysplasia, mitoses, or atypical cells.13 The tumor does not metastasize initially and is thus considered a low-grade SCC. However, we have observed foci of SCC in a perianal GC of short duration and small extension, and 30% to 50% of GC will transform into SCC later in the course of the disease.2,10,12

Human papillomavirus type 6 or 11 DNA is regularly found in verrucous carcinomas, strongly suggesting a pathogenic role in tumor development.14 These low-risk HPV types normally lack malignant potential, as they are found in benign CA but not in anogenital cancers, which usually contain DNA of high-risk HPV, mainly types 16 or 18. It remains unknown which viral or host risk factors, such as increased viral gene expression or inability to mount a cytotoxic immune response, may change the oncogenic potential of HPV types 6 or 11, causing progression of benign CA to the invasive GC phenotype.2

The literature about GC, which consists mainly of case reports, lacks controlled studies; therefore, treatment guidelines have not been established. An analysis based on 42 published cases concluded that the only consistently effective therapy is wide surgical excision of the tumor with clear margins, with or without adjunct chemotherapy.2 Other reported treatments include topical podophyllum resin, topical and systemic chemotherapy with fluorouracil, systemic bleomycin therapy in combination with cisplatin and methotrexate, laser excision, and tumor destruction using cryotherapy or electrocauterization.3,15 Radiotherapy has been used in severe cases of verrucous carcinoma and remains controversial, as progression to SCC with metastases has been reported.16 Regardless of the treatment modality, there is a high rate of local recurrence, resulting in an overall mortality rate of 20%.2

For the treatment of benign genital or laryngeal warts, which are caused by low-risk HPV-6/11, systemic, intralesional, or local application of interferon has demonstrated limited efficacy, mainly as adjunct to other established therapies, in decreasing the recurrence rate.1722 Possible effector mechanisms include antiviral or antiproliferative effects on HPV-infected cells and immunomodulatory activities.23

Interestingly, 2 case reports have previously described successful interferon monotherapy for GC. Systemically applied recombinant interferon alfa-2a (1.8 million units 5 days a week) in a woman with GC infiltrating the vagina, cervix, and bladder resulted in complete regression of the tumor after 6 months, but the long-term outcome was not reported.4 Intralesional injection of human recombinant interferon alfa-2b (9 million units 3 times a week) led to complete resolution of a GC after 5 months of continuous treatment,5 but lack of infiltration in that case makes the distinction from a large benign CA difficult.

A cell-mediated immune response plays an important role in the natural control of HPV infection. This is supported by the highly increased incidence of HPV-induced lesions in patients with iatrogenic or acquired cellular immunodeficiency, but not in those with humoral immune defects. CD4-positive lymphocytes and monocytes/macrophages dominate the cellular infiltrate in regressing genital warts, and there is a significant induction of the immune-accessory molecules HLA-DR and intercellular adhesion molecule 1 on keratinocytes.24 However, the viral or cellular determinants recognized in wart regression are unknown.

In our patient, after the clinical regression of the GC, a biopsy specimen from residual tissue showed a delayed-type hypersensitivitylike dense mononuclear cell infiltrate attached to and invading the overlying epithelium. Immunohistochemical analysis revealed a pattern dominated by CD3/CD4-positive cells. It has been suggested that major histocompatibility complex class I– restricted cytotoxic T cells are the principal effectors of protective immunity to noncytopathic viruses and tumors25,26 and that important mechanisms in the clearance of virus-induced lesions include the secretion of perforin and certain granzymes by cytotoxic cells.27 Also, our results corroborate the hypothesis that CD4-positive lymphocytes restricted by major histocompatibility complex class II elements may also exert cytotoxic function in recovery from viral infection.28

Because spontaneous regression of GC has not been reported to our knowledge, we can speculate that the long-term local injection of interferon had an immunostimulatory effect, possibly by inducing afferent, antigen presentation, as well as efferent, cytotoxic, immune mechanisms. Also, direct antiviral and antiproliferative effects of interferon may have contributed to tumor regression.

In our patient, malignant transformation was not observed in a large excisional biopsy specimen. Although we cannot formally exclude the initial presence of malignant foci elsewere, this fact might have contributed to the favorable outcome.

Previously reported failures of interferon to induce regression of GC6 or to prevent recurrences7 may be explained by host-specific factors, lower interferon dosages, or shorter treatment periods, as we have not observed tumor regression before 6 months of continuous therapy. Regression was complete after 12 months of treatment and has continued for a total of 28 months to date.

Controlled studies are required to determine whether interferon alfa as a monotherapy or as an adjunct to surgery is an effective treatment compared with surgery alone and to ascertain its potential in reducing the high incidence of tumor recurrence.

Accepted for publication January 18, 2000.

Reprints not available from the authors.

Tieben  LMBerkhout  RJSmits  HL  et al.  Detection of epidermodyplasia verruciformis-like human papillomavirus types in malignant and premalignant skin lesions of renal transplant recipients. Br J Dermatol. 1994;131226- 230
Chu  DCVezeridis  MPLibbey  NPWanebo  HJ Giant condyloma acuminatum (Buschke-Loewenstein tumor) of the anorectal and perianal regions: analysis of 42 cases. Dis Colon Rectum. 1994;37950- 957
Ilkay  AKChodak  GWVogelsang  NJGerber  GS Buschke-Loewenstein tumor: therapeutic options including systemic chemotherapy. Urology. 1993;42599- 601
Zachariae  HLarsen  PMSorgaard  H Recombinant interferon alpha -2a (Roferon-A) in a case of Buschke-Löwenstein giant condyloma. Dermatologica. 1988;177175- 179
Tsambaos  DMonastirli  AKapranos  NGeorgiou  SPasmatzi  EBerger  H Intralesional interferon alpha-2b therapy for Buschke-Loewenstein tumour. Acta Derm Venereol. 1994;74457- 459
Machens  HGRaab  RMaschek  HPichlmayr  R Giant condylomata acuminata (Buschke-Loewenstein tumor) of the rectum: a case report. Acta Chir Austriaca. 1995;271- 8
Grassegger  AHöpfl  RHussl  HWicke  KFritsch  P Buschke-Loewenstein tumour infiltrating pelvic organs. Br J Dermatol. 1994;130221- 225
Young  IRStark  DDedBradley  WG Jred Special pulse sequences and techniques. Magnetic Resonance Imaging. St Louis, Mo Mosby– Year Book Inc1988;84- 107
Buschke  ALöwenstein  L Über carcinomähnliche Condylomata acuminata des Penis. Klin Wochenschr. 1925;361726- 1728
Björck  MAthlin  LLundskog  BA Giant condylomata acuminatum (Buschke-Löwenstein tumor of the anorectum with malignant transformation). Eur J Surg. 1995;161691- 694
Schwartz  PA Verrucous carcinoma of the skin and the mucosa. J Am Acad Dermatol. 1995;321- 21
Creasman  CHaas  PAFox  TABalazs  M Malignant transformation of anorectal giant condyloma acuminatum (Buschke-Loewenstein tumor). Dis Colon Rectum. 1989;32481- 487
Lever  WFSchaumburg-Lever  G Diseases caused by viruses. Histopathology of the Skin. 7th ed Philadelphia, Pa JB Lippincott1990;399- 425
Gissmann  Lde Villiers  EMzur Hausen  H Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. Int J Cancer. 1982;29143- 146
George  AHahn  MD Carbon dioxide laser surgery in treatment of condyloma. Am J Obstet Gynecol. 1981;1411000- 1008
Perez  CAKraus  FTEvans  JCPowers  WE Anaplastic transformation in verrucous carcinoma of the oral cavity after radiation therapy. Radiology. 1966;86108- 115
LeventhaI  BGKashima  HKMounts  P  et al.  Long-term response of recurrent respiratory papillomatosis to treatment with lymphoblastoid interferon alfa-N1. N Engl J Med. 1991;325613- 617
Gross  GRoussaki  ABrzoska  J Low dose of systemically administered recombinant interferon gamma effective in the treatment of genital warts. J Invest Dermatol. 1988;90242
Boot  JMBlog  FBStolz  E Intralesional interferon alpha-2b treatment of condylomata acuminata previously resistent to podophyllum resin application. Genitourin Med. 1989;6550- 53
Gross  GRoussaki  APfister  H Die postoperative Interferon-Hydrogel-Behandlung. Hautarzt. 1988;39684- 687
Fierlbeck  GRassner  G Condylomata acuminata mit dem Nachweis von HPV-6-DNA. Hautarzt. 1989;40767- 770
Olsen  EAKelly  FFVollmer  RTBuddin  DAWeck  PK Comparative study of systemic interferon alpha-nl and isotretinoin in the treatment of resistant condylomata acuminata. J Am Acad Dermatol. 1989;201023- 1030
Condylomata International Collaborative Study Group, Recurrent condylomata acuminata treated with recombinant interferon alpha-2a. Acta Dermatol Venerol (Stockh). 1993;73223- 226
Coleman  NBirley  HRenton  AM  et al.  Immunological events in regressing genital warts. Am J Clin Pathol. 1994;102768- 774
Zinkernagel  RMHengartner  H Antiviral immunity. Immunol Today. 1997;18258- 260
van Driel  WJRessing  MEBrandt  RM  et al.  The current status of therapeutic HPV vaccine. Ann Med. 1996;28471- 477
Smyth  MJTrapani  JA The relative role of lymphocyte granule exocytosis versus death receptor–mediated cytotoxicity in viral pathophysiology. J Virol. 1998;721- 9
Kolatis  GDoymaz  MRouse  BT Demonstration of MHC class II–restricted cytotoxic T lymphocytes in mice against herpes simplex virus. Immunology. 1990;71101- 106

Figures

Place holder to copy figure label and caption
Figure 1.

Left, Clinical presentation. A large, exophytic, cauliflowerlike perianal verrucous tumor is seen extending toward the perineum and scrotum. Right, Dramatic regression of the tumor is evident after 12 months of treatment.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.

Magnetic resonance imaging scans (short-time inversion recovery technique). Top, Before treatment, the tumor is seen infiltrating the internal obturator muscle, ischiorectal fossa, and perirectal space (arrows indicate the lateral tumor border). Bottom, After 12 months of treatment, the internal obturator muscle is again well defined (arrow).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.

Top, Pretreatment histologic section shows papillomatous tumor invading deeply into the underlying tissue, without penetration of the basement membrane (hematoxylin-eosin, original magnification ×200). Bottom, The epidermis is hyperplastic and well stratified with few mitoses or koilocytes (hematoxylin-eosin, original magnification ×400).

Graphic Jump Location
Place holder to copy figure label and caption
Figure 4.

Histologic section of residual tissue after 12 months of treatment shows a bandlike mononuclear infiltrate in close approximation to and invading into the epidermis (hematoxylin-eosin, original magnification ×100).

Graphic Jump Location

Tables

References

Tieben  LMBerkhout  RJSmits  HL  et al.  Detection of epidermodyplasia verruciformis-like human papillomavirus types in malignant and premalignant skin lesions of renal transplant recipients. Br J Dermatol. 1994;131226- 230
Chu  DCVezeridis  MPLibbey  NPWanebo  HJ Giant condyloma acuminatum (Buschke-Loewenstein tumor) of the anorectal and perianal regions: analysis of 42 cases. Dis Colon Rectum. 1994;37950- 957
Ilkay  AKChodak  GWVogelsang  NJGerber  GS Buschke-Loewenstein tumor: therapeutic options including systemic chemotherapy. Urology. 1993;42599- 601
Zachariae  HLarsen  PMSorgaard  H Recombinant interferon alpha -2a (Roferon-A) in a case of Buschke-Löwenstein giant condyloma. Dermatologica. 1988;177175- 179
Tsambaos  DMonastirli  AKapranos  NGeorgiou  SPasmatzi  EBerger  H Intralesional interferon alpha-2b therapy for Buschke-Loewenstein tumour. Acta Derm Venereol. 1994;74457- 459
Machens  HGRaab  RMaschek  HPichlmayr  R Giant condylomata acuminata (Buschke-Loewenstein tumor) of the rectum: a case report. Acta Chir Austriaca. 1995;271- 8
Grassegger  AHöpfl  RHussl  HWicke  KFritsch  P Buschke-Loewenstein tumour infiltrating pelvic organs. Br J Dermatol. 1994;130221- 225
Young  IRStark  DDedBradley  WG Jred Special pulse sequences and techniques. Magnetic Resonance Imaging. St Louis, Mo Mosby– Year Book Inc1988;84- 107
Buschke  ALöwenstein  L Über carcinomähnliche Condylomata acuminata des Penis. Klin Wochenschr. 1925;361726- 1728
Björck  MAthlin  LLundskog  BA Giant condylomata acuminatum (Buschke-Löwenstein tumor of the anorectum with malignant transformation). Eur J Surg. 1995;161691- 694
Schwartz  PA Verrucous carcinoma of the skin and the mucosa. J Am Acad Dermatol. 1995;321- 21
Creasman  CHaas  PAFox  TABalazs  M Malignant transformation of anorectal giant condyloma acuminatum (Buschke-Loewenstein tumor). Dis Colon Rectum. 1989;32481- 487
Lever  WFSchaumburg-Lever  G Diseases caused by viruses. Histopathology of the Skin. 7th ed Philadelphia, Pa JB Lippincott1990;399- 425
Gissmann  Lde Villiers  EMzur Hausen  H Analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 DNA. Int J Cancer. 1982;29143- 146
George  AHahn  MD Carbon dioxide laser surgery in treatment of condyloma. Am J Obstet Gynecol. 1981;1411000- 1008
Perez  CAKraus  FTEvans  JCPowers  WE Anaplastic transformation in verrucous carcinoma of the oral cavity after radiation therapy. Radiology. 1966;86108- 115
LeventhaI  BGKashima  HKMounts  P  et al.  Long-term response of recurrent respiratory papillomatosis to treatment with lymphoblastoid interferon alfa-N1. N Engl J Med. 1991;325613- 617
Gross  GRoussaki  ABrzoska  J Low dose of systemically administered recombinant interferon gamma effective in the treatment of genital warts. J Invest Dermatol. 1988;90242
Boot  JMBlog  FBStolz  E Intralesional interferon alpha-2b treatment of condylomata acuminata previously resistent to podophyllum resin application. Genitourin Med. 1989;6550- 53
Gross  GRoussaki  APfister  H Die postoperative Interferon-Hydrogel-Behandlung. Hautarzt. 1988;39684- 687
Fierlbeck  GRassner  G Condylomata acuminata mit dem Nachweis von HPV-6-DNA. Hautarzt. 1989;40767- 770
Olsen  EAKelly  FFVollmer  RTBuddin  DAWeck  PK Comparative study of systemic interferon alpha-nl and isotretinoin in the treatment of resistant condylomata acuminata. J Am Acad Dermatol. 1989;201023- 1030
Condylomata International Collaborative Study Group, Recurrent condylomata acuminata treated with recombinant interferon alpha-2a. Acta Dermatol Venerol (Stockh). 1993;73223- 226
Coleman  NBirley  HRenton  AM  et al.  Immunological events in regressing genital warts. Am J Clin Pathol. 1994;102768- 774
Zinkernagel  RMHengartner  H Antiviral immunity. Immunol Today. 1997;18258- 260
van Driel  WJRessing  MEBrandt  RM  et al.  The current status of therapeutic HPV vaccine. Ann Med. 1996;28471- 477
Smyth  MJTrapani  JA The relative role of lymphocyte granule exocytosis versus death receptor–mediated cytotoxicity in viral pathophysiology. J Virol. 1998;721- 9
Kolatis  GDoymaz  MRouse  BT Demonstration of MHC class II–restricted cytotoxic T lymphocytes in mice against herpes simplex virus. Immunology. 1990;71101- 106

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