0
We're unable to sign you in at this time. Please try again in a few minutes.
Retry
We were able to sign you in, but your subscription(s) could not be found. Please try again in a few minutes.
Retry
There may be a problem with your account. Please contact the AMA Service Center to resolve this issue.
Contact the AMA Service Center:
Telephone: 1 (800) 262-2350 or 1 (312) 670-7827  *   Email: subscriptions@jamanetwork.com
Error Message ......
Case Report/Case Series |

Two Different Scenarios of Squamous Cell Carcinoma Within Advanced Basal Cell Carcinomas Cases Illustrating the Importance of Serial Biopsy During Vismodegib Usage FREE

Gefei A. Zhu, BS1; Uma Sundram, MD, PhD1,2; Anne Lynn S. Chang, MD1
[+] Author Affiliations
1Department of Dermatology, Stanford University School of Medicine, Redwood City, California
2Department of Pathology, Stanford University School of Medicine, Stanford, California
JAMA Dermatol. 2014;150(9):970-973. doi:10.1001/jamadermatol.2014.583.
Text Size: A A A
Published online

ABSTRACT

Importance  Vismodegib is a Hedgehog signaling pathway inhibitor recently approved by the US Food and Drug Administration for advanced basal cell carcinoma. We present 2 cases of clinically significant squamous cell carcinoma within the tumor bed of locally advanced basal cell carcinoma found during vismodegib treatment.

Observations  The first case is that of a patient with locally advanced basal cell carcinoma responsive to vismodegib but with an enlarging papule within the tumor bed. On biopsy, this papule was an invasive acantholytic squamous cell carcinoma. The second case is that of a patient with Gorlin syndrome with a locally advanced basal cell carcinoma that was stable while the patient was receiving therapy with vismodegib for 2.5 years but subsequently increased in size. Biopsy specimens from this tumor showed invasive squamous cell carcinoma, spindle cell subtype. In both cases, the squamous cell carcinomas were surgically resected.

Conclusions and Relevance  These cases highlight the importance of repeated biopsy in locally advanced basal cell carcinomas in 2 clinical situations: (1) when an area within the tumor responds differentially to vismodegib, and (2) when a tumor stops being suppressed by vismodegib. Timely diagnosis of non–basal cell histologic characteristics is critical to institution of effective therapy.

Figures in this Article

Vismodegib is a Hedgehog pathway inhibitor recently approved by the US Food and Drug Administration for treatment of advanced basal cell carcinoma (BCC).14 In animal models, decreased Hedgehog signaling has been shown to predispose to squamous cell carcinoma (SCC).5,6 Recently, 4 cases of vismodegib-associated SCC at sites distant to the BCC have been reported.79 Herein, we report a clinically important phenomenon of SCCs within the tumor bed of locally advanced BCCs, which became apparent after institution of vismodegib therapy. In both these cases, biopsies prior to vismodegib therapy showed basal cell histologic characteristics only, without indication of squamous cell differentiation.

REPORT OF CASES

Case 1

A woman in her 60s with no history of SCCs presented with a 2.8 × 3.4-cm ulcerated plaque involving the right medial canthus, upper eyelid, lacrimal duct opening, and nasal sidewall (Figure 1A). Owing to the extent of the tumor, 2 biopsy specimens were taken, 1 from the superior aspect of the tumor on the upper eyelid and 1 from the inferior aspect of the tumor on the cheek. Both biopsy specimens demonstrated infiltrative and nodular BCC with focal nonneoplastic epithelial hyperplasia (Figure 1B). A computed tomographic (CT) scan demonstrated erosion of the right nasal bone by tumor. The patient was referred for Mohs surgery but opted to pursue medical therapy with vismodegib owing to concern about surgical morbidity from the periocular location, lacrimal duct involvement, and invasion into nasal bone.

Place holder to copy figure label and caption
Figure 1.
Clinical Photographs and Histopathologic Findings of Case 1

A, Locally advanced basal cell carcinoma (BCC) affecting the right medial canthus and surrounding structures. Arrowheads indicate the areas of the tumor that were biopsied. B, Histopathologic findings demonstrated dermal nests of basaloid keratinocytes with peripheral palisading and retraction artifact, consistent with nodular BCC. Hematoxylin-eosin, original magnification ×200. C, An enlarging hyperkeratotic papule within the regressing BCC tumor bed during vismodegib therapy was biopsied (arrowhead). D, Histopathologic findings demonstrated atypical keratinocytes with focal acantholysis invading the dermis, consistent with an invasive acantholytic squamous cell carcinoma. Hematoxylin-eosin, original magnification ×200.

Graphic Jump Location

The patient started therapy with vismodegib, 150 mg daily, with dramatic tumor shrinkage. After 4 months of vismodegib, the patient reported enlargement of a discrete 0.5 × 0.7-cm hyperkeratotic papule within the prior BCC tumor bed (Figure 1C). Comparison with a pretreatment photograph showed that this papule was likely present, but smaller, prior to vismodegib treatment. A biopsy of the papule showed invasive acantholytic SCC and no abnormal basaloid cells (Figure 1D). The SCC was resected with Mohs surgery that required only 1 stage. The patient continued therapy with vismodegib during and after Mohs surgery, with subsequent complete response of the surrounding BCC.

Case 2

A woman in her 40s with Gorlin syndrome presented with a 15-cm, locally advanced, nodular BCC on the vertex of her scalp extending to the dura (Figure 2A and B). The BCC had previously been treated with photodynamic therapy and radiation, with disease recurrence. Owing to the advanced nature of her scalp BCC, she was started on therapy with vismodegib,150 mg daily. The scalp BCC shrank to 11 cm after 3 months of treatment with vismodegib. She continued to receive vismodegib over the next 2.5 years with stable scalp disease until a head magnetic resonance imaging (MRI) scan demonstrated tumor progression. Owing to the size of the tumor, 2 areas of the same tumor were biopsied (Figure 2C). Both biopsy specimens showed an atypical pleiomorphic spindle cell proliferation (Figure 2D). Immunohistochemical analysis was performed with positive staining for cytokeratin (CK) 5/6 (Figure 3A) and p63, supporting a diagnosis of keratinocytic neoplasm and ruling out atypical fibroxanthoma. Immunohistochemical staining of tumor cells showed that the cells did not stain with antibodies to CD10 (Figure 3B) and CD68 (Figure 3C), further ruling out a diagnosis of atypical fibroxanthoma; Sox-10 and S-100, ruling out melanoma; and smooth muscle actin (Figure 3D); and desmin, ruling out leiomyosarcoma. Taken together, these immunohistochemical findings support the diagnosis of invasive SCC, spindle cell subtype. There were no abnormal basaloid cells in the biopsy specimens.

Place holder to copy figure label and caption
Figure 2.
Clinical Photographs and Histopathologic Findings of Case 2

A, Locally advanced basal cell carcinoma (BCC) on the scalp prior to treatment. B, Histopathologic findings demonstrated nests of basaloid keratinocytes with peripheral palisading, consistent with nodular BCC. Hematoxylin-eosin, original magnification ×200. C, Same tumor after 2.5 years of vismodegib exposure. D, Histopathologic findings demonstrated invasive squamous cell carcinoma, spindle cell subtype. Hematoxylin-eosin, original magnification ×200.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.
Immunohistochemical Analysis of Advanced Basal Cell Carcinoma/Spindle Cell SCC in Case 2

A, Strong positive cytokeratin (CK) 5/6 staining suggested a keratinocytic neoplasm. Tumor cells did not stain with antibodies to (B) CD10, (C) CD68, or (D) smooth muscle actin (SMA). B and D, Arrowheads indicate tumor cells that were nonstaining. The SMA antibody weakly stained epithelioid myofibroblasts in the same section. E, Histopathologic findings from the excision specimen showed positive CK AE1/AE3/CAM5.2 staining of tumor cells on the left side of the image. Areas of basaloid cells on the right side of the image did not stain with CK AE1/AE3/CAM5.2. A-E, Original magnification ×200.

Graphic Jump Location

After consultation with the Stanford multidisciplinary head and neck tumor board, the patient underwent surgical resection of the scalp tumor, including removal of portions of the calvarium and dura. Frozen sections demonstrated discontinuous distribution of spindled cells with significant atypia and mitoses. These areas were interspersed with nodular and infiltrative BCC. Some of the spindled cells stained positive with an antibody cocktail for CK AE1/AE3/CAM5.2, whereas areas of transition between spindle and basaloid cells did not stain, consistent with dedifferentiation of a predominantly BCC tumor into discontinuous areas of spindle cell SCC (Figure 3E).

DISCUSSION

These cases illustrate 2 different scenarios of SCCs within advanced BCCs during the course of vismodegib usage. Case 1 is likely that of a collision tumor of an SCC and BCC, which did not become apparent until the BCC regressed while the patient was receiving vismodegib and the SCC did not. Case 2 is likely that of a BCC that dedifferentiated focally either before or during vismodegib therapy, particularly because Figure 3E shows a continuous transition from basaloid cells to spindle cells that are CK AE1/AE3/CAM 5.2 positive, without a clear border separating the 2. This second case prompted a biopsy after initial tumor shrinkage (likely of basaloid areas but not dedifferentiated areas) because it subsequently began to grow.

While there are no published data on the average time frame in which responses of locally advanced BCCs to vismodegib are detectable on clinical examination, our experience in treating dozens of patients1 has been that partial responses with tumor shrinkage are visible by 3 months. Hence, new or persistent ulcerations, nodules, or erythema in the locally advanced BCC tumor bed should prompt a biopsy after this time.

These cases highlight the importance of repeated biopsy in locally advanced BCCs in 2 clinical situations: (1) when an area within the tumor responds differentially to vismodegib and (2) when vismodegib stops suppressing tumor progression. Without repeated biopsies, these areas of tumor resistance10 might be incorrectly assumed to be BCC histologic characteristics; only a biopsy can lead to a correct and timely diagnosis of non-BCC histologic characteristics. Accurate diagnosis is critical to institution of potentially more effective therapy, such as surgical resection (as in these cases), chemotherapies specific for SCC (eg, capecitabine, cetuximab, erlotinib), or radiation.

CONCLUSIONS

Squamous cell carcinomas associated with vismodegib have been reported in sites separate from the advanced BCC being treated.79 In the clinical setting, new, growing lesions at a distant site would likely trigger a decision to biopsy owing to their location separate from the known advanced BCC. Our cases illustrate the need to rebiopsy the same tumor if the tumor (or portions of the tumor) grows after previous shrinkage while the patient is receiving vismodegib therapy.

The exact biologic mechanisms behind the 2 cases presented herein are intriguing but beyond the scope of this report. For instance, we speculate that the first case could be either (1) a collision tumor with the SCC present within the original locally advanced BCC, and/or (2) the SCC could arise owing to chronic inflammation from the larger BCC (akin to Marjolin ulcer). In the second case, we hypothesize that the SCC evolved from the BCC under the selection pressure of vismodegib, which favored the growth of cells resistant to vismodegib. Another explanation could be sampling error on the initial biopsy of a large BCC with preexisting areas of dedifferentiation, with regression of the basaloid cells on vismodegib favoring detection of the SCC on biopsy. Definitive proof of these alternative hypotheses would require mutational analysis of single tumor cells.

Finally, the existence of basosquamous tumors as a single entity continues to be debated, with theories including (1) collision between BCC and SCCs11 or (2) dedifferentiation of BCC into SCC.12 Our 2 cases illustrate that both may exist. Smoothened inhibition via vismodegib offers a unique opportunity to explore these possibilities in human keratinocytic tumors.

ARTICLE INFORMATION

Corresponding Author: Anne Lynn S. Chang, MD, Department of Dermatology, Stanford University School of Medicine, 450 Broadway St, Redwood City, CA 94063 (alschang@stanford.edu).

Accepted for Publication: March 7, 2014.

Published Online: April 16, 2014. doi:10.1001/jamadermatol.2014.583.

Author Contributions: Mr Zhu and Dr Chang had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.

Study concept and design: Zhu, Chang.

Acquisition, analysis, or interpretation of data: All authors.

Drafting of the manuscript: All authors.

Critical revision of the manuscript for important intellectual content: All authors.

Administrative, technical, or material support: All authors.

Study supervision: Chang.

Conflict of Interest Disclosures: Dr Chang has been a clinical investigator for studies sponsored by Lilly Infinity, Genentech, and Novartis. No other disclosures are reported.

REFERENCES

Chang  AL, Solomon  JA, Hainsworth  JD,  et al.  Expanded access study of patients with advanced basal cell carcinoma treated with the Hedgehog pathway inhibitor, vismodegib. J Am Acad Dermatol. 2014;70(1):60-69.
PubMed   |  Link to Article
Epstein  EH.  Basal cell carcinomas: attack of the Hedgehog. Nat Rev Cancer. 2008;8(10):743-754.
PubMed   |  Link to Article
LoRusso  PM, Rudin  CM, Reddy  JC,  et al.  Phase I trial of Hedgehog pathway inhibitor vismodegib (GDC-0449) in patients with refractory, locally advanced or metastatic solid tumors. Clin Cancer Res. 2011;17(8):2502-2511.
PubMed   |  Link to Article
Sekulic  A, Migden  MR, Oro  AE,  et al.  Efficacy and safety of vismodegib in advanced basal-cell carcinoma. N Engl J Med. 2012;366(23):2171-2179.
PubMed   |  Link to Article
Kang  HC, Wakabayashi  Y, Jen  KY,  et al.  Ptch1 overexpression drives skin carcinogenesis and developmental defects in K14Ptch(FVB) mice. J Invest Dermatol. 2013;133(5):1311-1320.
PubMed   |  Link to Article
Wakabayashi  Y, Mao  J-H, Brown  K, Girardi  M, Balmain  A.  Promotion of Hras-induced squamous carcinomas by a polymorphic variant of the Patched gene in FVB mice. Nature. 2007;445(7129):761-765.
PubMed   |  Link to Article
Iarrobino  A, Messina  JL, Kudchadkar  R, Sondak  VK.  Emergence of a squamous cell carcinoma phenotype following treatment of metastatic basal cell carcinoma with vismodegib. J Am Acad Dermatol. 2013;69(1):e33-e34.
PubMed   |  Link to Article
Aasi  S, Silkiss  R, Tang  JY,  et al.  New onset of keratoacanthomas after vismodegib treatment for locally advanced basal cell carcinomas: a report of 2 cases. JAMA Dermatol. 2013;149(2):242-243.
PubMed   |  Link to Article
Orouji  A, Goerdt  S, Utikal  J, Leverkus  M.  Multiple highly and moderately differentiated squamous cell carcinomas of the skin during vismodegib treatment of inoperable basal cell carcinoma [published online January 21, 2014]. Br J Dermatol. doi:10.1111/bjd.12840.
Chang  AL, Oro  AE.  Initial assessment of tumor regrowth after vismodegib in advanced basal cell carcinoma. Arch Dermatol. 2012;148(11):1324-1325.
PubMed   |  Link to Article
Maloney  ML.  What is basosquamous carcinoma? Dermatol Surg. 2000;26(5):505-506.
PubMed   |  Link to Article
Costantino  D, Lowe  L, Brown  DL.  Basosquamous carcinoma: an underrecognized, high-risk cutaneous neoplasm: case study and review of the literature. J Plast Reconstr Aesthet Surg. 2006;59(4):424-428.
PubMed   |  Link to Article

Figures

Place holder to copy figure label and caption
Figure 1.
Clinical Photographs and Histopathologic Findings of Case 1

A, Locally advanced basal cell carcinoma (BCC) affecting the right medial canthus and surrounding structures. Arrowheads indicate the areas of the tumor that were biopsied. B, Histopathologic findings demonstrated dermal nests of basaloid keratinocytes with peripheral palisading and retraction artifact, consistent with nodular BCC. Hematoxylin-eosin, original magnification ×200. C, An enlarging hyperkeratotic papule within the regressing BCC tumor bed during vismodegib therapy was biopsied (arrowhead). D, Histopathologic findings demonstrated atypical keratinocytes with focal acantholysis invading the dermis, consistent with an invasive acantholytic squamous cell carcinoma. Hematoxylin-eosin, original magnification ×200.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 2.
Clinical Photographs and Histopathologic Findings of Case 2

A, Locally advanced basal cell carcinoma (BCC) on the scalp prior to treatment. B, Histopathologic findings demonstrated nests of basaloid keratinocytes with peripheral palisading, consistent with nodular BCC. Hematoxylin-eosin, original magnification ×200. C, Same tumor after 2.5 years of vismodegib exposure. D, Histopathologic findings demonstrated invasive squamous cell carcinoma, spindle cell subtype. Hematoxylin-eosin, original magnification ×200.

Graphic Jump Location
Place holder to copy figure label and caption
Figure 3.
Immunohistochemical Analysis of Advanced Basal Cell Carcinoma/Spindle Cell SCC in Case 2

A, Strong positive cytokeratin (CK) 5/6 staining suggested a keratinocytic neoplasm. Tumor cells did not stain with antibodies to (B) CD10, (C) CD68, or (D) smooth muscle actin (SMA). B and D, Arrowheads indicate tumor cells that were nonstaining. The SMA antibody weakly stained epithelioid myofibroblasts in the same section. E, Histopathologic findings from the excision specimen showed positive CK AE1/AE3/CAM5.2 staining of tumor cells on the left side of the image. Areas of basaloid cells on the right side of the image did not stain with CK AE1/AE3/CAM5.2. A-E, Original magnification ×200.

Graphic Jump Location

Tables

References

Chang  AL, Solomon  JA, Hainsworth  JD,  et al.  Expanded access study of patients with advanced basal cell carcinoma treated with the Hedgehog pathway inhibitor, vismodegib. J Am Acad Dermatol. 2014;70(1):60-69.
PubMed   |  Link to Article
Epstein  EH.  Basal cell carcinomas: attack of the Hedgehog. Nat Rev Cancer. 2008;8(10):743-754.
PubMed   |  Link to Article
LoRusso  PM, Rudin  CM, Reddy  JC,  et al.  Phase I trial of Hedgehog pathway inhibitor vismodegib (GDC-0449) in patients with refractory, locally advanced or metastatic solid tumors. Clin Cancer Res. 2011;17(8):2502-2511.
PubMed   |  Link to Article
Sekulic  A, Migden  MR, Oro  AE,  et al.  Efficacy and safety of vismodegib in advanced basal-cell carcinoma. N Engl J Med. 2012;366(23):2171-2179.
PubMed   |  Link to Article
Kang  HC, Wakabayashi  Y, Jen  KY,  et al.  Ptch1 overexpression drives skin carcinogenesis and developmental defects in K14Ptch(FVB) mice. J Invest Dermatol. 2013;133(5):1311-1320.
PubMed   |  Link to Article
Wakabayashi  Y, Mao  J-H, Brown  K, Girardi  M, Balmain  A.  Promotion of Hras-induced squamous carcinomas by a polymorphic variant of the Patched gene in FVB mice. Nature. 2007;445(7129):761-765.
PubMed   |  Link to Article
Iarrobino  A, Messina  JL, Kudchadkar  R, Sondak  VK.  Emergence of a squamous cell carcinoma phenotype following treatment of metastatic basal cell carcinoma with vismodegib. J Am Acad Dermatol. 2013;69(1):e33-e34.
PubMed   |  Link to Article
Aasi  S, Silkiss  R, Tang  JY,  et al.  New onset of keratoacanthomas after vismodegib treatment for locally advanced basal cell carcinomas: a report of 2 cases. JAMA Dermatol. 2013;149(2):242-243.
PubMed   |  Link to Article
Orouji  A, Goerdt  S, Utikal  J, Leverkus  M.  Multiple highly and moderately differentiated squamous cell carcinomas of the skin during vismodegib treatment of inoperable basal cell carcinoma [published online January 21, 2014]. Br J Dermatol. doi:10.1111/bjd.12840.
Chang  AL, Oro  AE.  Initial assessment of tumor regrowth after vismodegib in advanced basal cell carcinoma. Arch Dermatol. 2012;148(11):1324-1325.
PubMed   |  Link to Article
Maloney  ML.  What is basosquamous carcinoma? Dermatol Surg. 2000;26(5):505-506.
PubMed   |  Link to Article
Costantino  D, Lowe  L, Brown  DL.  Basosquamous carcinoma: an underrecognized, high-risk cutaneous neoplasm: case study and review of the literature. J Plast Reconstr Aesthet Surg. 2006;59(4):424-428.
PubMed   |  Link to Article

Correspondence

CME
Also Meets CME requirements for:
Browse CME for all U.S. States
Accreditation Information
The American Medical Association is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The AMA designates this journal-based CME activity for a maximum of 1 AMA PRA Category 1 CreditTM per course. Physicians should claim only the credit commensurate with the extent of their participation in the activity. Physicians who complete the CME course and score at least 80% correct on the quiz are eligible for AMA PRA Category 1 CreditTM.
Note: You must get at least of the answers correct to pass this quiz.
Please click the checkbox indicating that you have read the full article in order to submit your answers.
Your answers have been saved for later.
You have not filled in all the answers to complete this quiz
The following questions were not answered:
Sorry, you have unsuccessfully completed this CME quiz with a score of
The following questions were not answered correctly:
Commitment to Change (optional):
Indicate what change(s) you will implement in your practice, if any, based on this CME course.
Your quiz results:
The filled radio buttons indicate your responses. The preferred responses are highlighted
For CME Course: A Proposed Model for Initial Assessment and Management of Acute Heart Failure Syndromes
Indicate what changes(s) you will implement in your practice, if any, based on this CME course.

Multimedia

Some tools below are only available to our subscribers or users with an online account.

1,554 Views
13 Citations
×

Related Content

Customize your page view by dragging & repositioning the boxes below.

Articles Related By Topic
Related Collections
PubMed Articles
Myths in the Diagnosis and Management of Orbital Tumors. Middle East Afr J Ophthalmol ;22(4):415-20.
Anti-PD-1 therapy in nonmelanoma skin cancer. Br J Dermatol Published online Apr 8, 2016;
Jobs